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Abstract

This study aimed to determine whether female rat strain Wistar with intramammary injection of benzopyrene can form breast cancer, whether there is a correlation between the time of tumor formation and the size of tumor with the category of tumor. This study used 35 female rats Wistar strain, 8-11 weeks. All rats had been intramammary injected with benzopyrene 3-5% at a dose of 50 mg/KgBW in 5 injections every 2 days. Swelling/lumps occur between 4-5 weeks to 17-18 weeks. The tumor was processed for Hematoxylin Eosin (HE) staining, and the results were viewed under a microscope with 400x magnification. There were 48.57% of rats that succeeded in forming tumors, with 34.28% being malignant phyllodes, 2,86% being metaplastic carcinoma, 2.86% being benign phyllodes, 2.86% being mesenchymal, and 5.71% being lymph gland. The results showed that the Spearman correlation between the time of tumor formation and the category of tumor had a value of p = 0.002 (p<0.05), r = 0.509. The correlation between the size of the tumor and the category of tumor is p = 0.009 (p<0.05), r = 0.435. There is a significant correlation between the time of tumor formation and the size of the tumor, with the category of tumor with a positive correlation of moderate strength. Rat strain Wistar with intramammary injection of benzopyrene can be an alternative study of breast cancer‎.

Keywords: Benzopyrene, Breast cancer model, Female rat Wistar, Intramammary injection

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