Diab M., Shaker O., Nassar Y., Abd El-Aziz G., El-Marzoki M.

Abstract

Background: When some oncogenes as human epidermal growth factor receptor and Topoisomerase IIA get overexpressed, they have been indicated to cause the development and growth of special aggressive kinds of breast cancer (BC). In recent years, these proteins have become an important biomarkers. Objective: This examination aimed at identifying the expression of Her2 and TopoIIA genes in both metastatic and non-metastatic groups of cancer breast patients. Subject and Methods: The current study was carried out on 230 participants who were divided into 3groups: Group I (control) which included 50 healthy subjects; Group II which included 40 patients of metastatic breast cancer cases; and Group III which contained 140 patients with non-metastatic breast. A detailed history taking and thorough physical and clinical examination, and 5 ml of blood were collected from each subject, and biochemical tests were done. Total RNA was extracted from whole blood using RNA extraction kit, and then reverse transcription was done followed by real time PCR. Results: The results of the current examination indicated that there was a remarkable correlation between control and non-metastatic cancer groups considering Her2 and TopoIIA expression (p<0.001*) & (p<0.001*) respectively, and between control and metastatic groups considering Her2 and TopoIIA expression (p<0.001*) & (p<0.001*), and also between each other. Conclusion: Her2 and TopoIIA gene expressions can be considered as the most significant diagnostic markers to diagnose non-metastatic, and predict the metastatic breast cancer.