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Abstract

This work is crucial for investigating the antiproliferative effect of a phenyl isoserine derivative. The action of the phenyl isoserine derivative was estimated utilizing the ex vivo rat aorta ring (RAR) assay, cytotoxicity assay on human breast cancer cell line MCF-7, gene expression analysis for Vascular endothelial growth factor (VEGF) by real-time PCR, ADME/Tox profiling, and molecular docking studies. The findings indicated that the phenyl isoserine derivative had considerable antiangiogenic action in a dose-dependent fashion in the RAR assay. The phenyl isoserine derivative had an inhibiting effect on MCF-7 cells. The phenyl isoserine derivative exhibited a decrease in VEGF gene expression as identified by quantitative real-time PCR. ADME/Tox experiments demonstrated appropriate pharmacokinetic characteristics and predicted how well the docked medication will attach to the protein and forecasted the binding affinity of docked medication. This work shows that the phenyl isoserine derivative markedly reduces angiogenesis and suppresses VEGF activity, which is advantageous for tumor therapy‎.

Keywords: Phenyl isoserine derivative, Antiangiogenic activity, Breast cancer cell MCF-7, Molecular docking, VEGF gene expression

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