× Current Issue Archive Submit Article
Conflicts of Interest Copyright and Access Open access policy Editorial Policies Peer Review Policy Privacy Statement Publishing Ethics
Editor in chief Associate Editors Advisory Board International Editors
Contact Us About Us Aim & Scope Abstracting And Indexing Author Guidelines Join As Editor
Views: 3140 Downloads: 582

Antibacterial activity, cytotoxicity, and phytochemicals screenings of binahong (Anredera cordifolia (Ten.) steenis) leaf extract


, , , ,
  1. Department of Oral Biology, Faculty of Dentistry, Maranatha Christian University, Bandung, West Java, Indonesia.
  2. Faculty of Dentistry, Maranatha Christian University, Bandung, West Java, Indonesia.

Abstract

Streptococcus mutans can cause caries in human teeth. Currently, people are starting to turn to herbal plants, one of which is the binahong plant known to be used as an antibacterial. Herbal plants must have biocompatibility properties with the cell in the body. To determine the antibacterial effectiveness of the ethanol extract of binahong leaves against S. mutans and to determine its toxic effect on fibroblast cells. The antibacterial activity of binahong leaf extract was evaluated by the agar diffusion method (Kirby-Bauer). The method used for the cytotoxicity test in this study is the MTS Assay method. The phytochemical screening test of the sample ethanol extract of binahong leaves contained secondary metabolites of tannins, flavonoids, saponins, and triterpenoids. The results showed that binahong can be used as an antibacterial with an average inhibition zone diameter of 14.513mm at a concentration of 100%. There was no cytotoxicity effect on fibroblast cells in vitro. The IC50 result of 983.88g/mL is safe to use as a limit value. Binahong plant which contains active biological components has significant antibacterial activity against S. mutans and a very low toxic effect on fibroblast cells, so it can be used and developed as an ingredient of natural antibacterial.



Keywords: Antibacterial, Binahong leaf, Caries, Cytotoxicity, Streptococcus mutans


How to cite this article:
Vancouver
Sugiaman VK, Pranata BMD, Susila RA, Pranata N, Rahmawati DY. Antibacterial activity, cytotoxicity, and phytochemicals screenings of binahong (Anredera cordifolia (Ten.) steenis) leaf extract. J Adv Pharm Educ Res. 2024;14(1):1-7. https://doi.org/10.51847/BXxQtsSl1s
APA
Sugiaman, V. K., Pranata, B. M. D., Susila, R. A., Pranata, N., & Rahmawati, D. Y. (2024). Antibacterial activity, cytotoxicity, and phytochemicals screenings of binahong (Anredera cordifolia (Ten.) steenis) leaf extract. Journal of Advanced Pharmacy Education and Research, 14(1), 1-7. https://doi.org/10.51847/BXxQtsSl1s
Citation Formats:

Related articles:
Most viewed articles:


Contact Meral


Meral Publications
www.meralpublisher.com

Davutpasa / Zeytinburnu 34087
Istanbul
Turkey

Email: [email protected]
Tell: +905344998991