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Thin layer chromatographic profiling and evaluation of analgesic activity of Psidium guajava leaf extracts in mice

Jaydeep Sarkar, Sujoy Pal, Sanjib Bhattacharya, Moulisha Biswas


Psidium guajava L. (Myrtaceae), commonly known as guava in English, is an evergreen small tree grown in tropics and subtropics and also cultivated commercially in India for its consumable fruits. The present study assessed the different solvent extracts of P. guajava leaf for thin layer chromatography (TLC) and also evaluated their analgesic potential by acetic acid induced writhing assay in Swiss albino mice. The chloroform extract yielded maximum spots in TLC, followed by methanol and petroleum ether extracts respectively. The entire test extracts exhibited significant analgesic activity. The methanol extract was found to be the most potent followed by the chloroform and petroleum ether extracts respectively. The present preliminary study demonstrated marked analgesic activity of P. guajava leaf in Swiss mice.

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