Antioxidant potential of underutilized parts of salam (Syzygium polyanthum) using DPPH and CUPRAC methods
Abstract
Antioxidants can combat free radicals that can cause degenerative diseases. Natural antioxidant agents can be found in salam (Syzygium polyanthum). Thus, the purpose of this study is to assess the salam's underutilized roots and twigs as a natural antioxidant source. The study also aims to identify and determine the flavonoid compound levels in the selected extract. Salam's antioxidant properties were demonstrated by the detection of flavonoids and phenolic substances by phytochemical screening. Using UV-visible spectrophotometry, the amounts of antioxidant activity, total flavonoid, and total phenolic were determined. The cupric ion-reducing antioxidant capacity (CUPRAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) techniques were used to measure antioxidant activity. Using high-performance liquid chromatography, the flavonoid components of the chosen extract were identified and quantified. The most antioxidant activity using DPPH was found in the ethanol of salam root extract, which included rutin, apigenin, and apigenin-7-O-glucoside molecules. It is possible to develop salam roots as a novel potential antioxidant agent source.
Keywords: Antioxidant, Flavonoid, Phenolics, S. polyanthum, Unused parts
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