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Bilnur Sayavush Kizi Mamedova , Kristina Yurievna Peterson , Marina Albertovna Gabrielyan , Alina Ruslanovna Blimgotova^*✉ , Ahdan Muratovich Shidaev , Liana Albertovna Kazharova , Zalina Borisovna Shaova , Roman Borisovich Zhukov

1. Department of Therapy, Pediatric Faculty, Stavropol State Medical University, Stavropol, Russia.
2. Department of Therapy, Faculty of Medicine, Ural State Medical University, Ekaterinburg, Russia.
3. Department of Dentistry, Faculty of Dentistry, North Ossetian State Medical Academy, Vladikavkaz, Russia.
4. Department of Food Technology, Faculty of Biotechnology, Don State Agrarian University, Persianovskiy, Russia.

Abstract

The activity of selenium nanoparticles on a SPEV-2 culture using propidium iodide (PI) was studied. Amon the range of studied concentrations, 32 and 320 µg/mL had cytostatic effects on SPEV-2 cells, while 3.2 and 32 µg/mL had cytotoxic effects. During the day, selenium nanoparticles caused apoptosis in cells at concentrations of 32 and 320 µg/mL. The superimposition of photographs under different microscopic modes of light scattering and fluorescence registration allowed us to describe cytomorphological indicators marking the process of cell death under the action of an extract inducing DNA damage in cells under in vitro conditions. The appearance of dark formations in the cytoplasm, fuzzy contours of the cells themselves, and their acquisition of a stellate shape or preservation of a rounded, but with the fragmentation of the cell membrane and leakage of contents, as well as formation of conglomerates by dead cells, were observed. A decrease in the intensity of the PI glow indicates the decay of the DNA of the nucleus. The index of the number of dead cells can serve as an index of cytotoxicity‎.

Keywords: Propidium iodide, Apoptosis, Proliferation, Nanotechnology

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