TY  - JOUR
T1  - Cytomorphological assessment of the effect of selenium nanoparticles on SPEV-2 oncovirus cells ‎
A1  - Bilnur Sayavush Kizi Mamedova
A1  - Kristina Yurievna Peterson
A1  - Marina Albertovna Gabrielyan
A1  - Alina Ruslanovna Blimgotova
A1  - Ahdan Muratovich Shidaev
A1  - Liana Albertovna Kazharova
A1  - Zalina Borisovna Shaova
A1  - Roman Borisovich Zhukov
JF  - Journal of Advanced Pharmacy Education and Research
JO  - J Adv Pharm Educ Res
SN  - 2249-3379
Y1  - 2024
VL  - 14
IS  - 1
DO  - 10.51847/n2D0qDLaQa
SP  - 84
EP  - 90
N2  - The activity of selenium nanoparticles on a SPEV-2 culture using propidium iodide (PI) was studied. Amon the range of studied concentrations, 32 and 320 µg/mL had cytostatic effects on SPEV-2 cells, while 3.2 and 32 µg/mL had cytotoxic effects. During the day, selenium nanoparticles caused apoptosis in cells at concentrations of 32 and 320 µg/mL. The superimposition of photographs under different microscopic modes of light scattering and fluorescence registration allowed us to describe cytomorphological indicators marking the process of cell death under the action of an extract inducing DNA damage in cells under in vitro conditions. The appearance of dark formations in the cytoplasm, fuzzy contours of the cells themselves, and their acquisition of a stellate shape or preservation of a rounded, but with the fragmentation of the cell membrane and leakage of contents, as well as formation of conglomerates by dead cells, were observed. A decrease in the intensity of the PI glow indicates the decay of the DNA of the nucleus. The index of the number of dead cells can serve as an index of cytotoxicity‎.
UR  - https://japer.in/article/cytomorphological-assessment-of-the-effect-of-selenium-nanoparticles-on-spev-2-oncovirus-cells-6ulhvyubmd2affc
ER  -