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Ilyas Rustamovich Nurmuhambetov , Darya Stanislavovna Prokopovich , Alexander Evgenevich Chernishev , Arina Andreevna Shorganova , Stanislav Vladislavovich Samarkin^*✉ , Tanzila Timurovna Magomadova , Amnat Islamovna Vadaeva , Siyana Bilalovna Masueva

1. Department of Therapy, Faculty of Medicine, Federal State Budgetary Educational Institution of Higher Education "Saratov State Medical University named after V. I. Razumovsky" of the Ministry of Health of the Russian Federation, Saratov, Russian Federation.

2. Department of Therapy, Faculty of Medicine, Federal State Budgetary Educational Institution of Higher Education "Chechen State University named after A. A. Kadyrov ", Medical Institute, Grozny, Chechen Republic, Russian Federation. 

3. 3Department of Therapy, Faculty of Medicine, Federal State Budgetary Educational Institution of Higher Education "Kabardino-Balkarian State University named after H.M. Berbekov”, Nalchik, Kabardino-Balkarian Republic, Russian Federation. 

Abstract

In regenerative medicine, to create tissue equivalents of the skin, great importance is paid to the study of the biocompatibility of scaffolds and skin cell culture. In the current scientific work, the dynamics of fibroblast cell cycle parameters in the in vitro system were evaluated by flow cytofluorometry in two experimental models. The first model is to evaluate the biocompatibility of fibroblasts with scaffolds under 2D and 3D cultivation conditions. The second model is an assessment of the biology of fibroblasts after exposure to UV radiation. The fibroblasts necessary for the study were isolated and cultured using mechanical dissociation of the tissue. Cell lines of the third passage were used in the experiment. To study the effect of ultraviolet irradiation on the parameters of the fibroblast cell cycle in vitro culture, cell lines were irradiated with a UV lamp. To analyze the biocompatibility of the fibroblast cell line with scaffolds, fibroblasts were seeded on two types of carriers: "G-DERM" and "Transwell". It was found that for two weeks of cultivation, high cell biocompatibility was observed in the G-DERM scaffold under 2D cultivation conditions. UV irradiation causes the cell cycle to stop as a compensatory adaptive process in response to the action of damaging radiation. Temporary exposure and dose-dependent exposure to UV radiation for 30 seconds do not have a pronounced lethal effect on cell cultures‎.

Keywords: UV radiation, Skin cells, Fibroblasts, Scaffold technologies, Cell cycle

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