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Analysis of flavonoid content and antioxidant activity of Curcuma caesia roxb grown in different geographical areas


, , ,
  1. Department of Pharmacy, Vocational School, Sebelas Maret University Surakarta, Indonesia.
  2. Department of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University Surakarta, Indonesia.
  3. Department of Chemistry, Faculty of Mathematics and Natural Sciences, Sebelas Maret University Surakarta, Indonesia.
  4. Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Sebelas Maret University Surakarta, Indonesia.

Abstract

Indonesia is a country with a tropical climate, making it home to a large number of flora and fauna species. One of them is the Curcuma caesia Roxb plant. The study aims to compare the effects of growing locations on flavonoid content and antioxidant activity of Curcuma caesia Roxb. The plants were taken from different locations in Central Java, namely Kemuning (866 asl) and Kebumen (28 asl). Black turmeric rhizome was extracted by the maceration method using ethanol solvent at room temperature for 3 days. Flavonoid content was identified using thin-layer chromatography, while compound levels were determined using the spectrophotometric method. Data were analyzed using a t-test in SPSS. The results of phytochemical screening of the flavonoid compound group using thin-layer chromatography produced a spot with an Rf value of 0.96. Flavonoid level was 22.1904 mg qe/g in the samples from the Kemuning location and 19.3929 mg qe/g in the Kebumen location. The IC50 value was 63.349 mg/ml in the samples from the Kemuning location and 82.8423 mg/ml in the samples from the Kebumen location. The results of the sample measurements in Kemuning and Kebumen produced soil pH of 6.50 and 6.63; temperature of 23.0 and 29.0 ; air humidity of 80.33% and 76.00%; and light intensity of 10,595 and 27,354 lux. It can be concluded that the growing location affects the flavonoid levels and antioxidant activity of Curcuma caesia Roxb.



Keywords: Flavonoid, Antioxidant, Kemuning, Kebumen


How to cite this article:
Vancouver
Rohmani S, Astirin OP, Marliyana SD, Handayani N. Analysis of flavonoid content and antioxidant activity of Curcuma caesia roxb grown in different geographical areas. J Adv Pharm Educ Res. 2024;14(4):69-75. https://doi.org/10.51847/yy1abuEXOj
APA
Rohmani, S., Astirin, O. P., Marliyana, S. D., & Handayani, N. (2024). Analysis of flavonoid content and antioxidant activity of Curcuma caesia roxb grown in different geographical areas. Journal of Advanced Pharmacy Education and Research, 14(4), 69-75. https://doi.org/10.51847/yy1abuEXOj
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