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Abstract

Curcuma caesia Roxb. is a rich source of bioactive compounds, including flavonoids, alkaloids, polyphenols, and tannins, which act as antioxidants. This research aims to examine and compare the antioxidant activity of n-hexane, ethyl acetate, and water fractions of Curcuma caesia Roxb. rhizome extracts originating from Kemuning, Central Java, Indonesia, using DPPH and ABTS free radical immersion methods. Curcuma caesia Roxb. rhizome was extracted using an ethanol solvent, and then fractionated with n-hexane, ethyl acetate, and water. The DPPH and ABTS techniques were then used to assess the fractions' antioxidant activity.  The wavelengths used for spectrophotometric measurements were 517 nm and 734 nm.  The IC50 value metric expresses antioxidant activity. The results of the antioxidant activity test showed that all concentrations had the potential to capture ABTS and DPPH free radicals, with IC50 values ​​for the n-hexane, ethyl acetate, and water fractions. Based on the two antioxidant testing methods, the highest results were found in the ABTS method. The results of the antioxidant activity test showed that all concentrations could capture ABTS free radicals, with IC50 values ​​for the n-hexane, ethyl acetate, and water fractions of 66.0604 ppm (strong), 40.4678 ppm (very strong), and 39.9784 ppm (very strong), respectively. Meanwhile, the IC50 values ​​in the DPPH method were respectively 52.1287 ppm (strong), 110.3383 ppm (moderate), and 98.15651 ppm (strong). The IC50 value of the positive control, with vitamin C as a comparison, showed very strong antioxidant activity‎.

Keywords: Curcuma caesia, Antioxidant, Fraction, DPPH, ABTS

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