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Abstract

Biological responses to exercise training are complex because almost all organs and systems are involved in interactions that lead to many adaptations at the genetic and metabolic levels. The purpose is to investigate the effect of eight weeks of high-intensity interval training with almond gum supplementation on the expression of HMGB1, Caspace3P53 and genes in the heart tissue of type 2 diabetic rats. 40 adult male rats divided into 5 groups of 8, control (CO), diabetes (CD), exercise (DE), supplement (DS), exercise and supplement (DES). Diabetes was induced with a single injection of 55 m/kg STZ. Training group started with 5 intervals of 30 seconds (90% vo2max) and 1 minute of active rest (40% vo2max) in the first week and ended with 12 intervals in the last week. The rats receiving the supplement also received the almond gum supplement by oral gavage for eight weeks. 24 hours after the last training session, the heart tissue of the mice was sampled. Data analysis was done using independent t-tests and one-way ANOVA at a significance level of P≥0.05. The interactive effect of HIIT exercise, along with almond gum supplement, had no significant effect on HMGB1 (p=0.634) and caspase3 (p=0.554) gene expression, but almond gum consumption and intense exercise had a significant effect on HMGB1 (P=0.001) and in Caspase3. Respectively, p=0.031 and p=0.009, each of which was effective on gene expression alone. However, the interactive effect of HIIT exercise and supplement consumption had a significant effect on P53 gene expression (p=0.045).