P. Keshaav Krishnaa, Lakshmi Thangavelu, Anitha Roy

Abstract

Introduction: The aim of the study was to estimate the anti-inflammatory activity of Caralluma Fimbrata on Raw 264 Macrophage cell lines enhanced with LPS which stands for lipo polysaccharide. Materials and Methods: The ethanolic extract of Caralluma Fimbrata was obtained from Green Chem Herbal Extract, Bangalore as a gift sample. Raw 264 macrophage cell lines were obtained from NCSS, Pune for the study. The sample was then studied along with the obtained cell lines to estimate the expression of nitrous oxide. Nitrous oxide being a known substance involved in inflammation. The gene expression of iNOS was also studied. Results: The effects of different concentrations of Caralluma Fimbrata was assessed and the nitrous oxide levels was estimated. It was observed that with increased concentration of the ethanolic extract of Caralluma Fimbrata the nitrous oxide Level was lowered and thus it is proven that Caralluma Fimbrata possesses anti-inflammatory properties. Conclusion: Caralluma Fimbrata extract possesses anti-inflammatory activity showed by significantly decrease in production of pro-inflammatory mediator NO and also by the suppression by the iNOS gene expression even when enhanced with LPS.