× Copyright Disclaimer Privacy Policy Author Guidelines Current Issue Archive Publishing Ethics Join As Reviewer Advertise Submit Article Abstracting And Indexing Editorial Board Contact Editor-in-Chief Scope of the Journal About the Journal


JAPER is indexed in SCOPUS

Views: 204 Downloads: 67

Ruta graveolens methanol extract, fungal-mediated biosynthesized silver nanoparticles, and their combinations inhibit pathogenic bacteria


, , , , , , , ,

Abstract

Silver nanoparticles (AgNPs) have been shown to be able to inhibit bacterial growth while presenting a negligible threat to the development of bacterial resistance and the toxicity of conventional silver compounds to human cells. Various concentrations of R. graveolens MetOH extract, both by itself and in combination with AgNPs made by Aspergillus flavus, were tested against several clinical isolates of Gram-positive and Gram-negative bacteria in the current study using disc diffusion methods and minimal inhibitory concentrations (MIC). Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Pseudomonas aeruginosa, Shigella sp., Staphylococcus aureus, and Staphylococcus epidermidis were the clinical bacteria types used in this research. Utilizing LC-MS, the chemical profile of the methanol extract of R.  graveolens was investigated. AgNPs were initially identified by the development of a dark brown tint, UV-Vis spectroscopy, images of TEM, and the ATR-IR analysis. AgNPs and R.  graveolens MetOH extract demonstrated synergistic effects on all of the examined microbes. Despite having the highest sensitivity to both the AgNPs and the R. graveolens MetOH extract when used separately (with inhibition zones of 16 and 19 mm, respectively), S. aureus has the lowest IFA values (0.10-0.13) when compared to the other bacterial strains, and no synergistic activity was shown. With a 1.25-fold rise in IFA, AgNPs and the R. graveolens MetOH extract had the strongest synergistic effect against S. epidermidis. By combining nanoparticles (NPs) with antibiotics or various types of medicinal plant extract, these results could be used to create and enhance novel antibacterial therapies as cutting-edge medications.


How to cite this article:
Vancouver
Husein NF, Al-Tarawneh AA, Al-Rawashdeh SR, Khleifat K, Al-Limoun M, Alfarrayeh I, et al. Ruta graveolens methanol extract, fungal-mediated biosynthesized silver nanoparticles, and their combinations inhibit pathogenic bacteria. J Adv Pharm Educ Res. 2023;13(2):43-52. https://doi.org/10.51847/H8saGkIapx
APA
Husein, N. F., Al-Tarawneh, A. A., Al-Rawashdeh, S. R., Khleifat, K., Al-Limoun, M., Alfarrayeh, I., Awwad, A. E., AlSarayreh, A. Z., & Al-Qaisi, Y. T. (2023). Ruta graveolens methanol extract, fungal-mediated biosynthesized silver nanoparticles, and their combinations inhibit pathogenic bacteria. Journal of Advanced Pharmacy Education and Research, 13(2), 43-52. https://doi.org/10.51847/H8saGkIapx
Citation Formats:


Contact SPER Publications


SPER Publications and Solutions Pvt. Ltd.

HD - 236,
Near The Shri Ram Millenium School,
Sector 135,
Noida-Greater Noida Expressway,
Noida-201301 [Delhi-NCR] India