TY  - JOUR
T1  - Ruta graveolens methanol extract, fungal-mediated biosynthesized silver nanoparticles, and their combinations inhibit pathogenic bacteria
A1  - Nedaa Fawzi Husein
A1  - Amjad Asri Al-Tarawneh
A1  - Shahed Rashad Al-Rawashdeh
A1  - Khaled Khleifat
A1  - Muhamad Al-Limoun
A1  - Ibrahim Alfarrayeh
A1  - Ahmad Eyal Awwad
A1  - Ahmad Za’al AlSarayreh
A1  - Yaseen Taha Al-Qaisi
JF  - Journal of Advanced Pharmacy Education and Research
JO  - J Adv Pharm Educ Res
SN  - 2249-3379
Y1  - 2023
VL  - 13
IS  - 2
DO  - 10.51847/H8saGkIapx
SP  - 43
EP  - 52
N2  - Silver nanoparticles (AgNPs) have been shown to be able to inhibit bacterial growth while presenting a negligible threat to the development of bacterial resistance and the toxicity of conventional silver compounds to human cells. Various concentrations of R. graveolens MetOH extract, both by itself and in combination with AgNPs made by Aspergillus flavus, were tested against several clinical isolates of Gram-positive and Gram-negative bacteria in the current study using disc diffusion methods and minimal inhibitory concentrations (MIC). Klebsiella pneumoniae, Escherichia coli, Enterobacter cloacae, Pseudomonas aeruginosa, Shigella sp., Staphylococcus aureus, and Staphylococcus epidermidis were the clinical bacteria types used in this research. Utilizing LC-MS, the chemical profile of the methanol extract of R.  graveolens was investigated. AgNPs were initially identified by the development of a dark brown tint, UV-Vis spectroscopy, images of TEM, and the ATR-IR analysis. AgNPs and R.  graveolens MetOH extract demonstrated synergistic effects on all of the examined microbes. Despite having the highest sensitivity to both the AgNPs and the R. graveolens MetOH extract when used separately (with inhibition zones of 16 and 19 mm, respectively), S. aureus has the lowest IFA values (0.10-0.13) when compared to the other bacterial strains, and no synergistic activity was shown. With a 1.25-fold rise in IFA, AgNPs and the R. graveolens MetOH extract had the strongest synergistic effect against S. epidermidis. By combining nanoparticles (NPs) with antibiotics or various types of medicinal plant extract, these results could be used to create and enhance novel antibacterial therapies as cutting-edge medications‎.
UR  - https://japer.in/article/ruta-graveolens-methanol-extract-fungal-mediated-biosynthesized-silver-nanoparticles-and-their-com-mwf8srnerkstmwi
ER  -