Views: 324 Downloads: 63

Ema Ratna Sari , Netty Suhatri , Friardi Ismed , Deddi Prima Putra^*✉

1. Faculty of Pharmacy, Andalas University, Padang 25163, Indonesia.
2. STIFI Bhakti Pertiwi 30128 Palembang, Indonesia.
3. Faculty of Pharmacy, Andalas University, Padang, 25163, Indonesia.
4. Laboratory of Biota Sumatera, Andalas University, Padang 25163, Indonesia.

Abstract

Sida retusa L. is a plant belonging to the Malvaceae family native to the tropical and subtropical plains grossing abundantly in Indonesia. Traditionally this plant is used for arthritis, asthma, cough, flatulence, colic, burning sensation, hemorrhoids, intermittent fever, and general debility. This study evaluates the chemical content in metabolite profiling, total polyphenolic contents, and in vitro antioxidant activity from Sida retusa L. leaves extract from West Sumatra, Indonesia. Metabolite profiling determination of Sida retusa L. extract using LC-MS/MS method, total phenolic and flavonoid contents, and antioxidant activity was determined by spectrophotometry. The results of the chromatogram interpretation revealed five main components of Sida retusa L. from West Sumatra were epoxypheophorbide A, scortechinone X, stigmastane-3β,5α,6β-triol, shidasterone B, and linolenic acid. Total phenolic and flavonoid contents from Sida retusa L. were 31.848 mg GAE/g and 12.242 mg QE/g). Sida retusa L. from West Sumatra had an antioxidant activity with IC₅₀ value 468,137 ± 13,319 µg/mL. Sida retusa L. contains various potential secondary metabolites and sources of polyphenols and antioxidants and is a promising source of Indonesian natural ingredients‎.

Keywords: Sida retusa L, Metabolite profiling, Polyphenolic, Antioxidant

Citation Formats: