Evaluation of genotoxicity and cytotoxicity of silver nanoparticles
The toxicological and hygienic assessment of the safety of nanoscale colloidal silver produced on a large scale by the modern nano industry is an important task because of its employment medicine. This work aimed to evaluate the mutagenic and cytotoxic properties of silver nanoparticles stabilized with polyvinylpyrrolidone. Silver nanoparticles stabilized with polyvinylpyrrolidone were synthesized for the experiment. The silver particles had a size of about 300 nm, but some of them turned out to be slightly smaller, about 80 nm. The absorption spectrum of UV/Vis electromagnetic waves displayed an absorption peak of 409 nm, which is typical for silver nanoparticles. The exposure time was 10 minutes. Control cultures were placed in saline without glutamate for 10 minutes. Cultures were then returned to the original nutrient medium and placed in a CO2 incubator for 4.5 hours. The excess of β-galactosidase activity over the control in PVP was 0.9±0.03 and 1.2±0.03, and in AgNPs+PVP – 0.6±0.38 and 0.6±0.12. MCF7-test showed that in the presence of silver nanoparticles, the cells acquired a spherical shape and did not show proliferation. AgNPs+PVP activated cell proliferation, which can enhance the wound healing effect.
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