Muhammad Faris , Budi Utomo , Asra Al Fauzi , I Ketut Sudiana , Sri Maliawan , Yoes Prijatna Dahlan , Oski Illiandri , Moh. Hasan Machfoed , Abdul Hafid Bajamal

Abstract

Acute spinal cord injury (SCI) is a health burden that affects daily function. Based on its prevalence, SCI becomes a major problem in the country. Inflammation is one of SCI property that stays as the main target for management in the last decade. ACTH_4-10PRO⁸-GLY⁹-PRO¹ (Met-Glu-His-Phe-Pro-Gly-Pro) has been known for its anti-inflammatory properties but remains unclear for SCI models. This experimental study was done on the SCI model of the Sprague-Dawley rat. Neutrophil was taken as an inflammatory marker from the spinal cord of the rat model on 3 and 6 hours of observation following injury. Statistical analysis was done using ANOVA comparison test with significance cut off of p < 0.05, CI 95%.

Six samples from each control and treatment group (normal saline 3 and 6 hours; ACTH_4-10PRO⁸-GLY⁹-PRO¹⁰ 3 and 6 hours) were observed for immunoreacted values of neutrophil staining to assess inflammatory reactions in spinal cord injury models. Statistical analysis continued with the Kruskal-Wallis test and found significant differences (p <0.001) between groups. Post-hoc tests were carried out using the Steel method. The most significant difference in Neutrophil levels was found in the ACTH_4-10PRO⁸-GLY⁹-PRO¹⁰ 6 hours group against normal saline 6 hours‎.

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