Sabah M. Salih, Nihad A. Jafar, Bashar Sadeq Noomi

Abstract

Investigation for most feasible, easy, and cheapest laboratory methods for diagnosis of mycoplasma infection is a worldwide need. The present study aimed to estimate the prevalence of Mycoplasma infection (Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS)) in poultries and evaluating three serological techniques, serum plate agglutination (SPA) technique, enzyme-linked immunosorbent assay (ELISA), and Hemagglutination inhibition (HI) technique with polymerase chain reaction (PCR) technique done in previous work. Serum samples (276) were collected from chickens with respiratory and articular diseases (202 and 74 respectively). A high detecting rate for MG (31.8%) and MS (19.5%) was observed using the SPA technique in comparison with other techniques. The sensitivity and specificity of the three techniques according to the PCR technique varied, 100% specificity recorded using ELISA and HI techniques. The current study concluded that the SPA technique is a more appropriate technique for detecting MG and MS. ELISA and HI techniques are highly specific. Although, a certain level of false-positive results can be expected in any test.