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Tatyana Ivanovna Sakhnenkova , Linda Ruslanovna Abdul-Kadyrova , Zara Alaudinovna Akhilgova , Aleksandra Andreevna Brovikova , Oleg Olegovich Markov , Anzhelika Arutyunovna Saribekyan , Rustam Magometovich Sampiev , Anton Andreevich Loginov

Abstract

In modern clinical practice, cell therapy is actively used for the treatment of degenerative diseases and malformations, burns, and injuries, as well as during dental and cosmetic operations. Thus, an urgent task is the development of new materials for tissue engineering, which implies, among other things, the study of the interaction of human cells with a three-dimensional synthetic matrix during the formation of tissues. In this scientific work, the regularities of attachment and proliferation of HeLa cells in the polymer of lactic and glycolic acids are evaluated, and some parameters reflecting the state of cells in the polymer and the development of oxidative stress in them are evaluated. It was found that the stereo ultrastructural organization of cells grown in the matrix did not differ from cells grown in vials; the dynamics of cell growth were close to the growth rate under standard conditions in vials; HeLa cells were not exposed to stress in the matrix during the entire cultivation period, which was confirmed by a stable level of intracellular glutathione and the absence of an antioxidant effect‎.

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