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Simultaneous estimation of Gallic acid, Curcumin and Quercetin by HPTLC method


Vinitkumar Y. Thakker , Vaishali N. Shah1 , Uravashi D. Shah1 , Manish P. Suthar

Abstract

There are number of Polyherbal formulation available for psoriasis but standardization is essential for ensuring quality control of Polyherbal formulation. The aim of this study was to formulate psoriasis tablets and to standardize it by HPTLC using gallic acid, curcumin and quercetin as a biomarker. Chromatography was performed on silica gel 60F254 percolated TLC plate using toluene: ethyl acetate: forminc acid (4.5:3.0:0.2, v/v/v) as solvent system. Densitometry scanning was performed under reflectanceabsorbance mode at 366 nm to quantify the spots. The Rf values of gallic acid, curcumin and quercetin are 0.40, 0.73 and 0.55 respectively. The total peak areas of the standards (gallic acid, curcumin and quercetin) and the corresponding peak areas of extracts were compared and the Gallic acid, curcumin and quercetin contents were estimated to be 1.860, 1.682 and 0.4693 % w/w. The HPTLC method was validated as per the ICH guideline and to be simple, precise, specific, sensitive and accurate and can be used for routine quality control of raw material as well as formulation containing any of these compounds.



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