Development and Validation of RPHPLC method of Tacrine Hydrochloride in Nanoemulsion gel
A simple, specific, precise and accurate RPHPLC method has been developed for determination of Tacrine hydrochloride in bulk and nanoemulsion gel using C18 column; 250 mm length, 4.6 mm internal diameter, 0.5μ particle size with UV visible detector (detection wavelength 243nm).Chromatographic separation was performed in an isocratic mode with mobile phase consisting of 0.05M triethylamine: acetonitrile (80: 20,); pH 3 using methanol as a diluent at a flow rate of 1.5 ml/minutes. Retention time was found to be 5.8 minutes. Linearity was established over a range of 20 to 160μg/ml. The method was rugged, robust with LOD of 0.00101μg/ml and LOQ of 0.0029 μg/ml. The percentage purity of tacrine hydrochloride in nanoemulsion gel was found to be 100.63%.
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