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Lienda Bashier Eltayeb

Abstract

Vancomycin-resistant enterococci (VRE) are widespread bacteria that emerged as major multi-resistant pathogens responsible for nosocomial infections, current study aimed to determine the molecular characterization of vanB genes among Sudanese hospitalized patients. A total of 107 samples were collected from hospitalized patients , Enterococcus species were isolated and identified using colonial morphology, Gram stain, and biochemical tests in conformity with standard microbiological techniques. Antimicrobials susceptibility of enterococci was tested to 8 antibiotics based on the modified Kirby-Bauer disc diffusion method on Muller Hinton agar. Resistance to the vancomycin (van B) gene was molecularly identified among vancomycin-resistant isolates using the conventions PCR method. Seventy-one (66.4%) of patients were colonized with enterococci, where 38.4% were diagnosed as Enterococcus faecalis while 28 % of patients were infected with Enterococcus faecium. Resistant of E. faecalis. to other anti-microbial includes (68.3%) to ampicillin, (87.8%) ciprofloxacin, (70.7%) to penicillin is resistant among, and (56.6%) to ampicillin, (53.3%) to ciprofloxacin, (80%) to penicillin, and 70 % to Penicillin is resistant among E. faecium, among whole isolated Enterococci 39 (54.9%) were VRE, Majority of vancomycin-resistant enterococci (VRE) 33 (84.6%) were multi-drug resistant to ciprofloxacin, penicillin, ampicillin, and chloramphenicol. PCR analysis of VRE samples showed that 12 (16.9%) enterococci isolates had the van B gene. The prevalence of VRE was significantly documented with a relative increase in the frequency of the van B gene which plays a crucial role in nosocomial infections, as a consequence, antibiotic resistance in enterococci to glycopeptide antibiotics is regarded as a threat to nosocomial infections‎.

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