Olga Yakovlevna Mezenova, Savelyeva Ekaterina Leonidovna, Kudaibergenova Aliya Kamalovna, Danshina Svetlana Dmitrievna, Kurbanova Madinat Nasrudinovna, Elena Andreevna Rogozina, Saeid Keshtkar

Abstract

Lovastatin and ethanol are two important compounds greatly used in therapies and medicines. We have developed and patented a new type of yeast Hanseniaspora opuntiae MK-460485, which is characterized by increased resistance to stress factors and a high ability to produce bioethanol. In this study, we considered the ability of this strain to produce ethanol under optimal conditions as well as the possibility and amount of lovastatin production by it. The present study showed that the studied species have a very good ability to produce lovastatin, especially in the presence of mashed potatoes (up to 112 mg/l). Additionally, to determine the optimal growth conditions for new yeast with the production of the maximum amount of bioethanol, the method of mathematical modeling was used. The statistical method of Plackett-Burman with the Minitab 18.1^© software determined the effective factors of ethanol production by the new strain and their ranges of variation: temperature - from 25 ° C to 42 ° C; molasses concentration (carbon source) in the nutrient medium - from 20 to 100%; the concentration of ammonium nitrate (source of nitrogen) is from 1 g/l to 5 g/l. The experiment plan for optimizing the ethanol production objective function was developed using the CCD method (Central Mix Design), software Design Expert 7.0.0^©. Optimal conditions were obtained for ethanol production by this strain: molasses concentration - 56.5%, ammonium nitrate concentration - 3 g/l, and temperature – 30.41 ° C. Under optimized conditions, bioethanol production by the new strain increased from 16% to 19.6%.