The potential healing effect of Kleinhovia hospita L. leaves extract on second-degree burns in rats
Abstract
One of the issues in medical research is the management of burn wounds. New wound healing chemicals are hailed for their anti-inflammatory, antibacterial, and antioxidant properties, which help to speed up the healing process. Kleinhovia hospita L. has been widely used as traditional medicine by the Komering tribe, in South Sumatra, Indonesia. This research aims to screen the anti-inflammatory and antioxidant activity of K.hospita L. leaf extracts in vitro and to determine the healing effect of K.hospita L. leaf extract on 2nd-degree burns in rats. The screening activity of anti-inflammatory was used in the human red blood cell membrane stabilization (HRBC) assay and the antioxidant capacity was assessed by ferric reducing antioxidant power (FRAP) assay. The 2nd-degree wounds were induced in 5 groups of 6 rats each. Group 1 as the second-degree burn model group, and groups 2-5 received standard drug (lanakeloid® dose 1g/kg BW), and ethanolic leaves extract of K.hospita L. with doses 250, 500, and 750mg/kg BW, respectively. Parameters observed included the percentage of burn healing recovery, time total recovery, and skin histopathology. The best result of anti-inflammatory and antioxidant activity is in the ethanolic extract of K.hospita L. with 85.46% stabilization and antioxidant capacity with an EC50 value is 71.49 ppm. The best wound healing effect is the rats treated with 750 mg/Kg BW ethanolic extract with 100% recovery on the 16th day. Histopathological features on the 14th day show epithelial and collagen formation. Group 5 shows the best epithelialization compared to the lanakeloid® group.
Keywords: Kleinhovia hospita L., HRBC, FRAP, Epithelial, Phytopharmacology
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