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Akhmad Endang Zainal Hasan , Dimas Andrianto , Husnawati Husnawati , Nisa Najwa Rokhmah , Raihan Rispahandi Rizki , Eny Ida Riyanti

Abstract

Soursop leaf endophytic fungi is an endophytic fungus with a symbiotic relationship inside the soursop leaf. It possesses higher anticancer activity compared to the leaves themselves. This research aims to determine the acute toxicity of ethyl acetate extract from soursop leaf endophytic fungi (Phomopsis sp) so that it can be referred for future research. The research used Sprague-Dawley rats divided into 6 groups. Each group was administered with 0, 5, 50, 300, 2000, and 5000 mg/kg body weight (BW) of extract in water, respectively. A lethal dose of 50% was obtained at 4198 mg/kg BW. Blood creatinine level, SGPT, and SGOT values were all within normal concentration in rats, those were 0.443-0.408, 59.4-83.4, and 161.0-222.8 mg/dL, respectively. The results of acute toxicity testing on SD rats obtained a lethal dose value that caused 50% death or LD50 from Phomopsis sp. is 4198,564 mg/kg BW. After 14 days, creatinine levels ranged from 0.443-0.408, SGPT ranged from 59.4-83.4, and SGOT values were in the range of 161.0-222.8. All values were within normal levels in rats. It is recommended to use ethyl acetate extract with a dose below 300 mg/kg BW extract‎.

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