Secondary metabolitm determination from Brebes shallot’s ethanol extract and its ethyl acetate fraction “Allium ascalonicum L.”
Shallot is a type of onion (Allium ascalonicum L). Brebes is one of the most extensively consumed horticultural crop commodities, and it includes medicinal secondary metabolites. Furthermore, Brebes shallots are high in flavonoids, which are the most important phytochemical in food and provide a wide range of biological advantages for humans. The purpose of this study was to analyze the secondary metabolites and flavonoids content in shallot from Brebes, Indonesia. Maceration extraction with 70% ethanol solvent and fractionation with ethyl acetate solvent was used in this study. The results of the secondary metabolite compound test qualitatively showed that the Brebes shallot Simplicia contains volatile oil compounds, saponin, tannin, flavonoids, and terpenoids, and TLC test with toluene mobile phase: Ethyl acetate (4:0.5) obtained one spot on quercetin with an Rf value of 0.372, On the shallot extract, there was one spot with an Rf value of 0.359, and three spots with Rf values of 0.205, 0.359, and 0.449 on the shallot fraction.
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