Suparat Jongjai , Jongkon Saising , Rawiwan Charoensub , Pravaree Phuneerub

Abstract

In Thai traditional textbooks, Morinda citrifolia. fruit is an antidote to vomiting and cure oral diseases. The current study aimed to develop the standardization parameters, chemical evaluation, and anti-microorganisms activities of M. citrifolia fruit extracts against oral microorganisms including Staphylococcus aureus, Streptococcus mutans, and Candida albican.  M. citrifolia fruit (MC) were collected from 15 sources throughout Thailand and then examined the pharmacognostic specification including macroscopic characteristics, microscopic characteristics, and physicochemical parameters. The volatile oils of Morinda citrifolia fruit (MCO) were determined by GC/MS. The antimicrobial activity of ethanol extract (MCE), water extract (MCW), and volatile oils (MCO) of M. citrifolia fruit were carried out by examining the inhibition areas using the agar well diffusion method, minimal inhibition concentration (MIC), and minimal bactericidal concentration (MBC). The outcome of the study revealed that Physico-chemical identification showing a loss on drying, total ashes, acid-insoluble ashes, and water content should be not more than 10.992, 10.115, 0.973, and 6.882% w/w, respectively. The volatile oil content, water, ethanol, and hexane soluble extractive values should not be less than 0.762, 31.8257, 18.0992, 3.4964 % w/w, respectively. GC-MS analyses of MCO showed the major compound was octanoic acid (84.103%) and hexanoic acid (7.183%). Based on antimicrobial activities, MCE and MCW cannot have antimicrobial activity in the oral cavity. However, MCO was potentially effective in suppressing microbial growth agiant S.aureus and S.mutans, and C. albicans. The volatile oil of M.citrifolia fruit may be a beneficial component of oral health care products or drugs for oral disease‎.

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