Perspective for using 2,6-dimethylpyridine-N-oxide to reduce the toxic effect of xenobiotics in mammals
This work aimed to study the isolated and subsequent effects of 2,6-Dimethylpyridine-N-oxide (Ivin) and 2,4-D-2-EHE on the survival of rats and the population characteristics of the total mRNA that implements molecular genetic processes in rats. The studies on Wistar Han (SPF) rats were performed. It was found that Ivin at doses of 13 and 0.013 mg/kg bw, corresponding to 1/100 and 1/100000 DL50, does not cause clinical signs of intoxication in rats. Ivin increases the level of hybridization in mRNA preparations from both the rat liver and blood cells (to a greater degree at a dose of 13 mg/kg bw than at a dose of 0.013 mg/kg bw). On the contrary, after the action of 2,4-D-2-EHE to rats at a dose of 540 mg/kg bw (corresponding to 1 DL50), pronounced intoxication is observed after 6 hours with the following 40% lethal of the animals. After intraperitoneal administration of Ivin to rats, followed (after 24 hours) by oral administration of 2,4-D-2-EHE at the same doses, the viability of rats is preserved, and the degree of hybridization of cytoplasmic mRNA with [ɑ-32P]-cDNA only slightly decreases at the end of the second day, and the third day, the general condition of the animals' body has returned to normal, there was also an increase in the degree of hybridization between the mRNA molecules isolated from the control rats and [ɑ-32P]-cDNA isolated from the experimental rats. Based on the results obtained, the possible molecular genetic mechanisms of action of the studied compounds are discussed.
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