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Harry Kurniawan Gondo

Abstract

Preeclampsia (PE) was still the main cause of maternal death in the world. Many efforts to prevent this however some studies report that PE had a high incidence mainly in rural areas. Hence, one needs to solve this problem using herbal remedies.  Recently, Moringa oleifera (MO) was reported to have health benefits as antioxidative properties. Its main bioactive isothiocyanate (ITC) which had been reported to have anti-inflammatory properties. However, it was not known its potency to prevent or treat PE. We conducted a study using human trophoblast cell culture. Trophoblasts are isolated from placental tissue. To induce PE, we use high glucose to induce inflammation. After confluent, cells were grouped into 2 treatment groups, namely (1) normal glucose (5 mM as negative control) and high glucose exposure of 33 mM (positive control), each group was divided into 2 subgroups, (a) without isothiocyanate treatment and (b) isothiocyanate treatment dose of 0.1; 0,2; 0.4 and 0.8 mg/ml. Next, each treatment is cultured in a 5% CO2 incubator at, a temperature of 37 °C for 3 days. The supernatant was collected and assay its cytokine using ELISA. The result shows that pro-inflammation cytokines such as IL-6, IL17, and STAT3 were significantly decreased and anti-inflammation cytokine IL-10 was significantly increased compared to the control group. Hence we concluded that the ITC of Moringa oleifera had alleviated inflammation in the PE model human trophoblast cell culture‎.

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