Abbas Mokarramat-Yazdi, Hossein Soltaninejad, Hadi Zare-Zardini, Fatemeh Shishehbor, Ashraf ‎Alemi, Farzaneh Fesahat, Fatemeh Sadeghian‎

Abstract

In this study, a new combination drug (BH) from herbal and animal materials was designed and ‎its anticancer activity was examined in experimental and animal models. The animal and herbal ‎sections of drug were originated from Bee, date syrup as well as grape syrup, respectively. ‎After drug preparation, anticancer (Human Colon Cancer (CaCO2) Cell Line), anticoagulant, ‎and hemolysis activity were evaluated by MTT, coagulometer, and RDA methods, respectively. ‎Two different doses of the drug were tested on rats with colon tumor in the 18-day period by ‎determination of the size and weight of the tumor. The mice were killed after 18 days and their ‎three important tissues (heart, kidney, and liver) were histologically evaluated. The drug has ‎shown an effective anticancer effect on cancer cells, so that this drug completely prevents the ‎growth of cancer cells at a low concentration within 72 hours and the percentage of live cells ‎approximately reached zero after 72 hours. This drug has a more limited effect on normal cells ‎with proper selectivity (Toxicity on normal cells was 32%). The study of hemolysis activity on ‎red blood cells showed that the drug had no hemolytic activity on RBCs. This drug did not ‎effect on the coagulation system (PT and PTT pathways). The extent of the drug's ability to ‎differentiate between cancerous and normal cells was almost constant at a time interval of 24, ‎‎48, and 72 hours. In treatment group, the weight and size of the tumor significantly decreased ‎compared with the control group. However, there was no significant difference in comparison ‎with the chemotherapy drug, but even in this group, the tumor size and weight reduction was ‎higher in the new drug group. Histological data showed that both the new drug and the 5FU ‎drug resulted in necrosis of the tumor cells. Necrosis was 75% for 5FU and 90% for the new ‎drug. The new drug has no symptoms on the normal tissues of the body. In general, this drug ‎can distinguish between cancerous and normal cells with a specific mechanism, and its formula ‎is patterned from traditional medicine during extensive research. This drug can introduce as ‎new complementary drug for cancer treatment‎‎‎.