Abstract

Due to its geographical distribution problem, tissue culture propagation can be used for genetic resource and commercialization of Hypericum perforatum L. known as St John's wort, regarding its active ingredients such as hypericin, which is anti-AIDS and antidepressant. In this research, the way of callus induction and genetic resources were carried out on Hypericum perforatum L. by four explants, including root, stem, leaf, and hypocotyl. The explants were cultured in Murashige and Skoog (MS) medium along with two growth regulators, namely 2, 4-D at four hormonal levels (0.1, 0.2, 0.3, 0.4 mg/L) and BAP (0.25, 0.5, 1, 2 mg/L) in 16 hormonal treatments based on completely randomized design with 4 replications and each replicate containing 5 explants. According to data (callus number and diameter, dry weight, relative frequency) in each Petri, the highest amount of callus and genetic resource was achieved with 0.5 mg/L BAP and 0.1 mg/L 2, 4-D in root explant, and the lowest amount was at 0.5 mg/L  BAP and 0.2 mg/L 2, 4-D in stem explant. Generally, a high hormonal concentration of BAP resulted in a decreased number of callus, relative frequency, and callus diameter. Furthermore, a high hormonal concentration of 2, 4-D caused an increased number of callus, relative frequency, descending diameter, dry weight