Abstract

New extraction methods, such as ultrasonic and microwave extraction, are fast and effective methods for extracting effective compounds. From plant tissues. Ultrasound waves are soundwaves with frequencies above 20 kHz that create mechanical oscillations in a solid, liquid or gas in this study, the response surface method was used to determine the optimal points of the extraction process by ultrasonic method to achieve maximum phenolic  compounds and extract efficiency and the effect of factors such as frequency and temperature on the extraction of phenolic compounds, extract efficiency and anti- activity. In this method, we extracted the extracts of three medicinal plants Humulus lupulus, Malva sylvestris and Thymus in a water. Then, using GC-MS device, we investigated their chemical compounents. In Humulus lupulus, the maximum percentage efficiency with 31.15% is related to 1,2- Benzenedicarboxylic acid and in Malva sylvestris and Thymus plants, it is related to 46.23% respectively and 10.53% is related to Dimethylphosphinic acid and Methyl .beta-d-galactopyranoside.