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Abstract

This research endeavors to explore the correlation between the sperm DNA fragmentation index (DFI) and recurrent miscarriage in women seeking fertility treatment. 81 participants who experienced recurrent miscarriages from March 2020 to March 2022 and had their sperm DFI measured were selected from infertility centers in Ahvaz, Iran, and compared to the control group. Sperm volume, count, motility, and morphology were assessed. Sperm DNA fragmentation was examined using the TUNEL method and flow cytometry. Demographic information from patient records and the results of TSH, Anti-TPO, FSH, and AMH tests were collected and evaluated. The average age of males and females in the two groups with a history of miscarriage and those without showed a significant difference (p<0.05). Furthermore, this study found a higher prevalence of irregular menstrual cycles in the group with a history of miscarriage compared to the group without it (p<0.05). Furthermore, no significant differences were observed between the BMI of males and females, alcohol consumption, smoking habits, and drug usage in the two groups. Regarding laboratory variables, TSH and DFI levels were higher in the group with a history of miscarriage, while sperm counts, progressive motility, and morphology were higher in the group without miscarriage (p<0.05). However, the two groups had no significant differences in Anti-TPO, FSH, and AMH levels.

Investigating paternal factors such as sperm counts, volume, motility, morphology, and DFI is critical in assessing recurrent miscarriages in women. These factors can contribute to diagnostic improvements‎.