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Differential derivative method development and Validation of Orlistat by UV: A Spectrophotometric Technique

Priyanka Gaddam , Dhanalakshmi K , G. Nagarjuna Reddy S. Sreenivasa


The aim of the present research work was to develop accurate and rapid Differential derivative spectrophotometric method. The stock solution was prepared by taking methanol as solvent. Further dilutions were made by using equimolar solutions with 0.1N NaOH and 0.1N HCl separately and the difference in absorptivities was measured at wavelengths of 214 nm. It was proved that spectrum differentiation significantly corrects errors resulting from overlapping background. Difference in absorbance between maxima and minima was calculated to find out the amplitude. The amplitude was plotted against concentration. Beer’s-Lambert’s law is valid in the concentration range 10-30 for zero, first and second orders. The result of analysis was validated statistically and by recovery studies.

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