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Development of validated analytical methods for the simultaneous estimation of biomarkers in the leaves of Barleria cristata and bio analysis of the selected marker in plasma


Jayaprakasam Rajendran , Liji Jacob , RaviThengungal Kochupappy

Abstract

Two simple accurate and economical HPTLC and RP-HPLC methods were developed for the simultaneous estimation of biomarkers in Barleria cristata and bio analysis of the selected marker in plasma was also carried out. The successive extraction of leaves of Barleria cristata was carried out by using soxhlet apparartus. In the case of standardization as well as bioanalysis by HPTLC, the separation was achieved by using methanol: ethyl acetate: toluene: formic acid (1:1: 7.5: 0.2 %v/v/v/v) as the mobile phase. The detection was made at 308nm. In the case of RP-HPLC, the separation was achieved by using potassium dihydrogen phosphate buffer : methanol (30:70%v/v) and the detection wavelength used was 330nm. The proposed methods were validated as per ICH guidelines and the bio analytical method was validated as per USFDA guidelines. The methods were accurate, precise and rapid and found to be suitable for the quantitative analysis of plants and formulation containing phytochemical markers.




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