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Development and validation of UV Spectrophotometric method for simultaneous estimation of Lamivudine and Efavirenz in the Pharmaceutical dosage form


Manikanta Kumar A , B. Naga Sandhya , Mahesh Nasare , V.V.L.N Prasad , Prakash.V.Diwan

Abstract

A simple, specific, accurate and precise UV spectrophotometric method has been developed for simultaneous estimation of lamivudine and efavirenz in pharmaceutical dosage form. The absorption maxima of the drugs were found to be at 271 and 247 nm for lamivudine and efavirenz respectively. Lamivudine and efavirenz obeyed Beer’s law in the concentration range of 10-100 μg/ml and 10-70 μg/ml respectively. Different analytical parameters such as linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ) were determined as per ICH guidelines. Limit of detection and quantification values for lamivudine 10 and 3.5 μg/ml and for efavirenz 10 and 3.0 μg/ml respectively. The recovery values between prescribed limit of 98-102% shows that method is free from interference of excipients present in formulation. The developed method was free from interferences due to excipients present in formulation and it can be used for routine quality control analysis.




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