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Development and Validation of RP-HPLC method for the Simultaneous estimation of Ciprofloxacin Hydrochloride and Ornidazole in Combined Pharmaceutical Dosage Form

J. Ramya Krishna , B. Naga Sandhya , Sanayaima Huidrom , V.V.L.N Prasad


A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of Ciprofloxacin hydrochloride and Ornidazole in pure and combined pharmaceutical dosage form. The quantification of the drug was carried out using Reverse phase phenomenex® Luna 5μ C18 (2) 100A (250 × 4.60 mm i.d) column in isocratic mode with mobile phase containing Acetonitrile and Water (pH adjusted to 2.5 with O-phosphoric acid) in the ratio of (75:25 % v/v) was used. The flow rate was 1ml/min and the effluents were monitored at 289nm. The retention times of Ciprofloxacin hydrochloride and Ornidazole were found to be 6.319mins and 2.944 mins respectively. The developed method was validated in terms of linearity, accuracy, precision, LOD, LOQ as per ICH guidelines. Due to its simplicity, accuracy and high precision the proposed RP- HPLC method can be used for the simultaneous estimation of both the drugs in its combined pharmaceutical dosage form.

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