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Development and validation of RP-HPLC method for the estimation of Cefadroxil Monohydrate in bulk and its tablet dosage form

K. Govinda Rao , B. Uma Shankar , B. Phanindra , B. Mahesh Naik


A new simple and precise reverse phase high performance liquid chromatographic method has been developed and subsequently validated for the estimation of cefadroxil monohydrate in bulk and its pharmaceutical dosage form. The chromatographic separation was performed by using mobile phase consisting of KH2PO4: acetonitrile in the ratio of 65:35 % v/v and the pH 3.5 adjusted with 0.2% orthophosphoric acid. The column used was Hypersil ODS C18 (250 × 4.6 mm, 5μ) with flow rate of 1 ml/min using PDA detection at 220 nm. The described method was found to be linear over the range of 20-80μg/ml and correlation coefficient was found to be 1 with a retention time of 3.257min. The assay of cefadroxil was found to be 99.98 %. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise, reliable, accurate and economical which is useful for the routine determination of cefadroxil monohydrate in bulk and its pharmaceutical dosage form.

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