× Author Guidelines Publishing Ethics Join As Reviewer Advertise Current Issue Archive Submit Article Abstracting And Indexing Editorial Board Contact Editor-in-Chief Scope of the Journal About the Journal

JAPER is indexed in SCOPUS

Development and validation of a HPLC analytical assay method for amlodipine besylate tablets: A Potent Ca+2 channel blocker

Richa Sah and Saahil Arora


Amlodipine besylate is a potent calcium channel blocker used for the treatment of hypertension, congestive heart failure and angina pectoris. Amlodipine besylate avoids the adverse effect of amlodipine in racemic mixtures. A highly precise and cost effective RP-HPLC method with retention time of 2.60 minutes was developed for the estimation of amlodipine besylate in tablet dosage form, by fixing the parameters as WATERS C18 column 250 mm × 4.6 mm (5μm), with mobile phase as acetonitrile: 70mM potassium dihydrogen orthophosphate buffer: methanol (15:30:55) and pH adjusted to 3.0 using OPA. Mobile phase flow rate was maintained at 1.0 ml/min and detected at 240nm.

Contact SPER Publications

SPER Publications and Solutions Pvt. Ltd.

HD - 236,
Near The Shri Ram Millenium School,
Sector 135,
Noida-Greater Noida Expressway,
Noida-201301 [Delhi-NCR] India