Detection of Toxocara canis infection by ELIZA, with follow-up some Biochemical and histological changes
This study examined 100 blood samples from people visited General Hospital of Samarra and the external medical laboratories in Samarra city whose ages ranged between 6-38 years from October 2019 to March 2020 to investigate toxocariasis. 100 fecal samples were examined for the presence of intestinal parasite infections to avoid any cross-reaction with toxocariasis in the ELISA test. The study included knowledge of changes occurring in the levels of GOT and GPT, as well as knowledge of histological findings in animals experimentally infected with Toxocara canis parasite for each the liver, lung, spleen, kidney, and brain. The groups of animals were divided into two groups, euthanized at 7 and 14 days postinfection and necropsied for taking tissue sections to find the differences between the effects of infection on the somatic tissues. The results showed the number of positive serum samples was 13(13%), The highest number of infected people was 9 and the highest antibody value was 14.6 units compared to the control group (10 units) and was within the age group of 6-16 years. The biochemical tests showed an increase in the concentration of GOT and GPT enzymes in infected people with T. canis parasite compared with control. The highest concentration was recorded in the age group 17-27 and 6-16 years for both GOT and GPT (30.67±4.72 and 32.79±4.18 (IU/L), respectively). The histological study showed the presence of histological changes in each of the members that were studied and for each of the two groups 7 and 14 days postinfection.
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