× Author Guidelines Publishing Ethics Join As Reviewer Advertise Current Issue Archive Submit Article Abstracting And Indexing Editorial Board Contact Editor-in-Chief Scope of the Journal About the Journal


JAPER is indexed in SCOPUS

Detection of Toxocara canis infection by ELIZA, with follow-up some Biochemical and histological changes


Maroof Sabti Juma Al-Ammash, Lima Tariq Youash Lazar, Ali Khudheyer Obayes, Kasim Sakran Abass

Abstract

This study examined 100 blood samples from people visited General Hospital of Samarra and the external medical laboratories in Samarra city whose ages ranged between 6-38 years from October 2019 to March 2020 to investigate toxocariasis. 100 fecal samples were examined for the presence of intestinal parasite infections to avoid any cross-reaction with toxocariasis in the ELISA test. The study included knowledge of changes occurring in the levels of GOT and GPT, as well as knowledge of histological findings in animals experimentally infected with Toxocara canis parasite for each the liver, lung, spleen, kidney, and brain. The groups of animals were divided into two groups, euthanized at 7 and 14 days postinfection and necropsied for taking tissue sections to find the differences between the effects of infection on the somatic tissues. The results showed the number of positive serum samples was 13(13%), The highest number of infected people was 9 and the highest antibody value was 14.6 units compared to the control group (10 units) and was within the age group of 6-16 years. The biochemical tests showed an increase in the concentration of GOT and GPT enzymes in infected people with T. canis parasite compared with control. The highest concentration was recorded in the age group 17-27 and 6-16 years for both GOT and GPT (30.67±4.72 and 32.79±4.18 (IU/L), respectively). The histological study showed the presence of histological changes in each of the members that were studied and for each of the two groups 7 and 14 days postinfection.


How to cite this article:
Vancouver
Al-Ammash MSJ, Lazar LTY, Obayes AK, Abass KS. Detection of Toxocara canis infection by ELIZA, with follow-up some Biochemical and histological changes. J Adv Pharm Educ Res. 2021;11(1):182-8. https://doi.org/10.51847/Y8rnMNeEnQ
APA
Al-Ammash, M. S. J., Lazar, L. T. Y., Obayes, A. K., & Abass, K. S. (2021). Detection of Toxocara canis infection by ELIZA, with follow-up some Biochemical and histological changes. Journal of Advanced Pharmacy Education and Research, 11(1), 182-188. https://doi.org/10.51847/Y8rnMNeEnQ
Citation Formats:


Contact SPER Publications


SPER Publications and Solutions Pvt. Ltd.

HD - 236,
Near The Shri Ram Millenium School,
Sector 135,
Noida-Greater Noida Expressway,
Noida-201301 [Delhi-NCR] India