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Etis Duhita Rahayuningtyas , Irna Sufiawati , Ronny Lesmana , Muhammad Nur

Abstract

Oral squamous cell carcinoma (OSCC) is the most common type of oral cancer, approximately 90-95% of the total malignancy in the oral cavity. Eradicating cancer cells with radiation and chemotherapy have been concerned as cancer treatment methods, that tend to reduce immune system levels and activity. Ozone therapy has been developed to treat cancer without affecting the immune system. The objective of the study is to determine the cytotoxic effect of ozone and ozone combined with cisplatin against OSCC CAL-27 culture cells. OSCC CAL27 cells were cultured in 96 well plates. The MTS method is used as a cytotoxic test with DMEM as a media control, DMEM and cells as a negative control, cisplatin as a positive control, ozone dissolved in the media, and a combination of ozone and cisplatin as a treatment group whose dose is determined (500, 750, 1000, 1125, 1500, 3125, 3750 mg/mL). The double experiment was assessed with 4 replicates in each experiment. The corrected absorbance value was used to assess the effect of cytotoxicity. ANOVA statistical tests in experiments 1 and 2 showed a p-value <0.01 single-dose ozone, and a p-value <0.05 in a combination of ozone and cisplatin. The Pearson correlation test shows a strong correlation (0.711) in the first experiment and a very strong correlation (0.812) in the second experiment. Ozone has a cytotoxic effect on OSCC CAL-27 culture and ozone combined with cisplatin has a higher cytotoxic effect than single-dose ozone, so it can be considered a cancer adjuvant therapy‎.

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