Abstract

Although A low sperm concentration less than 20 million/ml and very little motility (less than 20%) is indicative of the risk of fertility, pregnancy sometimes occurs with these very small amounts. There are some methods by which the quality of sperm can be increased for inoculation. Two methods, mainly considered as laboratory techniques for improving the quality of sperm, include Swim-Up (SU) and density gradient centrifugation (DGC). The aim of the present study is to compare  the effect of these two methods on the quality of semen samples  in different groups, including normal samples  (< 60 million and 20-60 million/ml), oligospermia, asthenospermia, teratospermia, and oligoasthenoteratospermia in patients referred to the Infertility Center (ACECR).The present experimental study was performed on 1132 these samples were collected after 3 to 5 days abstinence, the findings of pre and post-preparation motility and count parameters were studied and compared in different groups. the SU method led to the highest improvement in the sperm count in the first normal group (20-60 million/ml).). the DGC-SU method led to the best improvement in the sperm count in the teratospermia group. the sperm motility in different groups show, the highest improvement in sperm motility was observed in the asthenospermia group  and the oligoasthenoteratozoospermia group in the post- preparation phase using SU and DGC-SU methods compared with other groups (P<0.01).The results showed that SU method was more effective than DGC-SU method in improving sperm count and motility.