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Brine shrimp lethality assay of Sesbania grandiflora ethanolic extract – in vitro study

Priyangaa Sathasivam , T. Lakshmi


To evaluate the lethality assay of Sesbania grandiflora using in vitro parameter. Brine shrimp lethality bioassay is a standard method to investigate the cytotoxicity of herbal extract. S. grandiflora commonly known as Agathi in Tamil that appears to have historical usage as an antibacterial and anti-inflammatory agent. A total of 20 nauplii were added into three replicates of each concentration of the plant extract. After 24 h, the surviving brine shrimps were counted, and percentage of mortality was assessed. This study reveals that S. grandiflora leaf possesses significant cytotoxic activity when tested in vitro. From this study, it can be concluded that ethanolic extract of S. grandiflora possessed marked cytotoxic effect.

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