Abstract

Hyperlipidemia is a major risk factor for the development of atherosclerosis and kidney disease progression. Rosuvastatin drug is used in the treatment of hypercholesterolemia. In this study, dispersive liquid-liquid microextraction based on solidification of floating organic drop (DLLME-SFO) combined with UV-Vis spectrophotometry Net Analyte Signal (NAS) was developed for the analysis of rosuvastatin in real samples. In this technique, 1- undecanol and ethanol were used as the extraction solvent and dispersive solvent, respectively and various parameters affecting extraction efficiency were optimized such as the type and volume of the extraction solvent disperser solvent, pH and extraction time. In the optimal condition, the linear calibration range was 0.034-0.517 µg ml^-1 and the correlation coefficient was 0.9998. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.0069 µg ml^-1 and 0.0231 µgml^-1, respectively. Moreover, the relative standard deviation of rosuvastatin was obtained 1.474 % for six replicates. Regarding spiking experiments on real samples, which was found by this method, was between 98.5 % and 110%. As a result, this method was successfully applied to the determination of the rosuvastatin in the pharmaceutical samples‎‎.