Biresh K Sarkar, Ritesh Patel, Upendra Bhadoriya

Abstract

Different Pyrazoline derivatives were synthesized by cyclization of substituted chalcone derivatives in presence of hydrazine hydrate (P1-P6). All the synthesized compounds were characterized by spectral analysis (IR, MS and NMR). These compounds were screened for their antibacterial activity against Gram-positive bacteria and Gram negative bacteria. Antifungal activity was also performed using agar cup plate method. The minimum inhibitory concentrations (MICs) of the compounds were also determined by agar streak dilution method. Among the synthesized compounds; (P1) was found to exhibits the most potent in-vitro antimicrobial activity with the MICs of 3.121, 1.5, 22 6g/ml against E. coli, P. aeruginosa, B. pumilus respectively. Compound (P6) was found to exhibit the most potent in-vitro anti-fungal activity with MICs 0.83 and 0.093 6g/ml against A. niger and P. chrysogenum.