Anti-diabetic activity of Sesbania grandiflora - alpha amylase inhibitory effect
Introduction: Diabetes mellitus is characterised by high blood sugar levels caused due to ineffectiveness of the insulin produced at the peripheral tissues. Due to the side effects of insulin and oral hypoglycaemic agent, demand to use natural products with anti-diabetic activity has been increased recently. Materials and Methodology: Sesbania grandiflora extract used in the study is obtained from Green Chem Herbal Extracts and Formulations, Bengaluru, India. Acarbose, Dinitrosalicylic acid, DMSO is obtained from Sigma Aldrich Co. India. α-Amylase enzyme were procured from HiMedia, Mumbai. In vitro amylase inhibition was studied by the method of Bernfeld and the percentage inhibition of α-Amylase enzyme was calculated. Results: A maximum inhibition of 81% was observed at 1000μg/ml which was compared with that of standard Acarbose that showed 93% inhibition. The IC50 of the extract was found to be 50.95μg/ml and for Acarbose 34.83 uM. Conclusion: The study showed that this plant species exhibited anti-diabetic property and can be used to explore possibilities of developing a natural drug for the management of diabetes mellitus rather than using synthetic drugs which produce side effects.
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