Analytical Standardization of Ayurvedic Formulation- Aqueous extracts of Hedichium spicatum Ham.Ex Smith, Sassurea lappa C.B.Clarke, Emblica officinalis Gaertn and Curcuma longaLinn
Ayurvedic Formulation is a quadric herbal aqueous extracts combination of Hedychium spicatum Hamsmithex, Sassurea lappa C.B.Clarke, Emblica officinalis Gaertn and Curcuma longa Linn. in equal proportions has been standardized by physicochemical, organoleptic, estimation of phytochemical compounds and HPTLC finger printing as for the guidelines of Ayurvedic pharmacopeia of India and WHO. The ingredients were authenticity by the standard markers and HPTLC finger prints. Physicochemical parameters shows values of pH 4.53, loss on drying 6.88%, water soluble extractive 87.97%, alcoholic soluble extractive 40.15%, total ash 18.96%, acid. insoluble ash 0.36%, heavy metal estimation of Lead <5ppm,Cadmium <1 ppm, Arsenic <2 ppm Phytochemical compound quantification assessed for curcuminoids by spectrometric, tannis by volumetric and total alkaloids by gravimetric methods shows the values of 0.27%, 15.12% and 0.24% respectively. The designed formulation was in conformity to the properties evaluated for clinical trial on tropical pulmonary eosinophilia in filaria. HPTLC finger printing shows 14 peaks with values of the Total height 902.2, Total area 23988.2 and Rf value of formulation observed between 0.02-0.65. HPTLC finger printing employed to fix standards which had given accurate, sensitive and specific quality control of raw material as well as formulation containing any of these compounds.
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