Abstract

Waddlia chondrophila is an intracellular bacterium that causes miscarriage in human and cattle. Overall, 50% of cases of miscarriage and recurrent miscarriage is unknown. Obviously, miscarriage for an unknown reason can be influenced by an unknown infectious agent. Researches shows that at least 2 percent of recurrent miscarriages are also directly related to infectious agents. However, no information is available in respect to the presence of this bacterium in Yazd. Due to the low sensitivity of cell culture to recover such an obligate, molecular-based diagnostic approaches are necessitated. This research investigates the development of a quantitative SYBR Green assay which targets the recA gene of W. chondrophila.