M.R. Izadpanah, Z. Farahnejad, A. Akbari‎‎‎‎

Abstract

Background : Todays,  using the various derivatives of thiosemicarbazone is increasing for cancer treatment. Due to the increasing rate of cancer, and in particular breast cancer in developing countries, the use of nanoparticles such as iron oxide magnetic nanoparticles to enhance the effect, as well as the targeted effect of various anticancer compounds such as thiosemicarbazone is of particular importance. Methods: In this study, the anticancer activity of thiosemicarbazone pyrazole derivative alone (compound P) and in conjunction with iron oxide nanoparticles (compound PL) was investigated on MCF-7 cell line. Following MTT assay to evaluate the toxicity of the P and PL compounds on MCF-7 cells, the RT-qPCR reaction was performed to measure the expression of BAK-1, EIF4E and hTERT genes in 24-hr treatment of MCF-7 cells with the above mentioned compounds. Finding: The results showed more strong anticancer activity of the PL compound on MCF-7 cells compared to the P compound. The IC50 values were obtained 15.780 and 13.184 μg/well for the P and PL compounds, respectively. Also, the effect of PL compound on the expression of targeted genes was Meaningfully more than P compound. Conclusion: Due to the high penetration capability of iron oxide nanoparticles, as well as the high toxicity of these compounds, the synthesis of magnetic P nanoparticles is used in the purposeful transfer of these compounds, in addition to increasing the toxicity of these compounds on cancer cells.